The Identification of QTL Associated with Cotton Fruit Branch Length Suitable for Mechanized Harvest Utilizing BSA-seq

[Objective] This study aims to identify quantitative trait loci (QTLs) associated with cotton fruit branch length by using the combination of bulked-segregant analysis (BSA) and next-generation sequencing (NGS) method. [Method] In this study, the cotton F₂ segregating population was developed with the short branch line Sujimian 125 and long-branch variety Sikang 1 as cross parents. According to the average internode length of fruit branches in middle, extreme individuals in F₂ population were divided into two groups to generate the bulked DNA samples. The whole-genome resequencing of two DNA bulks was applied to analyze the single nucleotide polymorphism (SNP) and insertion-deletion (InDel) between two groups, and the fruit branch internode length related QTLs were detected using Euclidean distance (ED). [Result] With SNP data obtained from sequencing, a 0.8 Mbp range associated region on chromosome A3 was identified; with InDel data obtained from sequencing, a 1.09 Mbp range associated region on chromosome A3 was identified. Two regions overlapped, and the overlapped range was 0.77 Mbp. [Conclusion] This result suggested that the difference between I-type fruit branch and zero-type was due to different genetic locus and pattern rather than dosage effect of the same gene.     

甘蓝型油菜DH群体抗倒伏相关性状遗传分析

为明确油菜抗倒伏性的遗传规律，本文利用抗倒伏性差异显著的2个甘蓝型油菜品系配制杂交组合，构建含280个株系的DH群体，采用作物茎秆强度抗倒测量仪等考种工具对该群体进行连续两年的抗倒伏性鉴定，并利用植物数量性状的主基因 + 多基因混合遗传模型及偏度和峰度分析对抗倒伏性进行遗传分析。结果表明，茎秆抗折力和茎秆抗折强度都受到0对主基因 + 9对微效多基因控制，茎秆抗折力和单株生物量的基因间无互作，茎秆抗折强度的基因间有互补作用，茎秆抗折力和茎秆抗折强度两年的平均遗传率在50%左右，而茎秆直径两年的平均遗传率为69.338 %，单株生物量两年的遗传率分别为52.198%和69.284%，遗传率都较低，性状受环境影响都较大，因此对于茎秆抗折力、茎秆抗折强度、茎秆直径和单株生物量，在育种选择的早期阶段都不宜太严格。同时茎秆抗折强度相比茎秆抗折力更能说明作物的抗倒伏能力，因而在抗倒伏选择育种时应更加关注。     

Length‐based assessment of larval lamprey population structure at differing spatial scales

1. Perspectives on lamprey management contrast between pest control in the US Great Lakes and species conservation in the Pacific Northwest, New Zealand, and Europe. Five lamprey species are listed in the European Union (EU) Habitats Directive (HD) as requiring conservation measures. Assessments of HD ‘conservation status’ for these lampreys mainly target the larval (ammocoete) stage.
2. Larval lamprey populations can be assessed by evaluating presence or absence, density and demographic structure in riverine samples. Demographic structure has typically been described from length–frequency data using qualitative approaches and the statistical assignment of age classes. Length‐based indicators (LBIs) may provide a more rapid and flexible framework.
3. The demographic structure of a fish population can be described by univariate length metrics: length range (L_RANGE) and 90^th percentile of length (L₉₀). This study used a pooled data set from seven Irish catchments to estimate a reference point (RP) value for each metric corresponding to a healthy larval lamprey population. Two LBIs were then derived that can be estimated for an observed (OBS) population as LBI_RANGE = L_{RANGE_OBS}/L_{RANGE_RP} and LBI₉₀ = L_{90_OBS}/L_{90_RP}.
4. Simulated lamprey population length structures, representing a range of status values, were used to develop a reference gradient (RG) to support population assessment using LBIs. The assessment framework was applied to each of the seven Irish catchments, and also to subcatchment and sampling‐site scales within the Barrow system. The LBIs and RG suggested that most catchments had favourable conservation status overall, whereas smaller assessment scales revealed a spread of status values.
5. The proposed framework allows the rapid assessment of demographic structure and comparison across systems. There is potential to track states across sampling sites, events, and legislative reporting cycles, and to interpret change in relation to the local environment. The general approach may be adaptable for other fish species monitored during juvenile stages.

     

不同新型作用机制杀菌剂对梨树主要病菌毒力及防治流程应用

 为明确多种新型作用机制杀菌剂与引起梨树褐斑病、黑星病、白粉病等病原菌的有效对靶关系及制定梨树主要病害防治流程,采用菌丝生长速率法、离体叶片法与田间药效方法研究新型杀菌剂对靶标病原菌的毒力、对靶标病害的防效及其田间有效应用,建立替代梨树主要病害传统化学杀菌剂的防治流程技术。结果显示,双胍三辛烷基苯磺酸盐在离体叶片法下对褐斑病的防效大于85%,田间药效验证3次用药后7 d防效大于85%、30 d防效大于80%、90 d防效仍大于60%,兼治轮纹病菌其毒力EC₅₀值均小于1 μg·mL^-1。双胍三辛烷基苯磺酸盐、辛菌胺醋酸盐、吩嗪α-2羧酸在离体叶片法下对黑星病防效大于90%且毒力EC₅₀值小于1 μg·mL^-1;田间药效验证1次用药后7 d防效大于80%、30 d防效仍大于75%。噻肟菌酯、硝苯菌酯、丙硫菌唑在离体叶片法下对白粉病的防效大于85%;田间药效验证3次用药后7 d铲除效果大于70%、30 d仍大于60%。丙硫菌唑对黑斑、轮纹病菌毒力EC₅₀值均小于1 μg·mL^-1;吩嗪α-2羧酸同时对褐斑、黑斑、轮纹病菌毒力EC₅₀值小于1 μg·mL^-1。针对梨树主要病害发生期,选用新型杀菌剂替代传统杀菌剂制定防治技术流程,其综合防效达到88.94%。不同新型作用机制杀菌剂在防治梨树主要病害上的应用,能够降低抗药性产生,同时达到有效防治的目的。     

Repeated annual drought has minor long‐term influence on δ13C and alkane composition of plant and soil in model grassland and heathland ecosystems

As a result of global climate change the incidence of drought conditions in Europe is predicted to increase in the future, which also influences plant resistance. Lipids are important plant constituents that protect plants against drought stress and contribute to the intermediate stable carbon (C) pool in soil. However, the extent to which drought influences lipid cycling in the plant–soil system is unknown and, therefore, it remains questionable how the ecosystem recovers after drought. We focused on plant and soil samples from two different plant communities (temperate grassland and heathland) that had been exposed to 5 years of 4.5–6.0 weeks repeated annual drought. They were sampled one year after the last drought to check the recovery of the plant–soil system. Samples were analyzed for their bulk C, stable C and nitrogen (N) isotope (δ¹³C, δ¹⁵N) and lipid composition. Contrary to our expectation, no strong influence of five years of repeated annual drought was observed for above‐ground biomass, roots and soils in the model ecosystems with respect to elemental (C and N concentrations, C : N ratio) bulk isotope (δ¹³C, δ¹⁵N) composition and the total extractable lipid concentration. Thus, plants did not sustain a significant change in their C and lipid concentration as well as their composition after five years of repeated annual drought. This might be related to the comparatively short drought period related to the overall growth season and provides evidence for recovery of the C and lipid dynamics in temperate grassland and heathland model ecosystems exposed to annual drought.     

不同生育期水稻干物质量与产量的关系研究

为明确不同生育期的干物重对粤晶丝苗2号、粤农丝苗、合美占3个水稻(Oryza sativa L.)品种的产量及产量构成因素的影响,设置了早造和晚造两次不同氮肥梯度随机区组栽培试验。结果表明,叶片干物质的分配比例在拔节孕穗期时最高,且其随着生育进程逐渐降低,在乳熟期达到最低值;茎干物质的分配比例在乳熟期降至最低;齐穗期的穗干物质分配比例不到20%,而乳熟期的穗干物质量将近占总干物质量的43%;早造和晚造水稻的结实率差异极显著,总干重差异显著,而穗数、每穗粒数、千粒重、产量差异不显著;齐穗期以后,结实率和千粒重均与干物重呈负相关,而叶干重与穗数呈显著正相关,且相关性较大,茎干重与各产量性状密切相关,总干重与产量呈正相关。水稻干物重是决定高产的重要因素。     

多尺度DEM提取河流比降的对比分析

研究旨在用DEM计算河流比降,分析DEM分辨率与阈值对比降的影响,并提取最佳分辨率与最佳阈值。以延河流域的ASTER GDEM和SRTM3 DEM为原始数据源,利用Arc GIS分别提取60、150、200、300 m分辨率下延河流域的主河道,并据此计算流域平均比降。结果表明:延河的平均比降为3.39‰;DEM分辨率与汇流阈值对比降影响较大,当DEM分辨率小于150 m时,比降随阈值增大而减少;DEM分辨率大于150 m,比降逐步增大。延河流域河流比降分析的DEM分辨率不宜超过150 m,其中DEM分辨率为90 m时,计算的比降结果较为符合延河流域实际情况;DEM分辨率为30、60、90、150、200、300 m时,计算比降的最佳阈值分别为65000、60000、30000、70000、40000、20000;利用85%的主河道折线段信息,进行比降计算,其结果接近真实值。研究实现了基于DEM的河流比降计算,有助于DEM在水文学等流域的应用。     

基于茎秆直径微变化的拔节期玉米水分亏缺诊断指标研究

为了探索利用茎秆直径微变化诊断玉米水分亏缺状况的最优指标,采用桶栽的方法,开展了不同水分处理条件下拔节期夏玉米茎秆直径微变化中的日最大收缩量(MDS)、日增长量(DI)和当日恢复时间(RT)3个指标的变化规律及其与土壤相对含水率之间相关关系方面的试验研究。结果表明,(1)不同处理间的MDS和DI受水分亏缺影响差异较为明显,RT对土壤相对含水率的响应较为一致;(2)玉米日最大收缩量MDS和日增长量DI受到环境因素的影响较大,与土壤相对含水率θ的相关系数较小;(3)恢复时间RT与土壤相对含水率θ的相关系数最高,且受水分亏缺处理的影响较小,可以作为诊断玉米水分亏缺状况的较好指标。综上可知,与MDS和DI相比,RT更适合诊断夏玉米水分亏缺状况。     

泾惠渠灌区畦长对夏玉米耗水特性和产量影响

泾惠渠灌区中畦田规格参差不齐,灌区由于畦田过长而造成的灌溉水量浪费屡见不鲜,不仅没有形成高产而且极大的造成了水资源的浪费。通过大田与小区试验相结合,以当地农民习惯的灌溉方式为对照,设置3个灌溉畦长处理(80、120、240 m),每个处理设置3次重复,改水为80%。研究了灌溉畦长对夏玉米在生育期内的耗水特性和产量的影响,土壤水分分布与产量及其构成因素的关系等问题,选择出兼顾节水高产可行的最优畦长。研究结果表明:在生育期灌水后,80和120 m处理畦长内畦首、畦中和畦尾的土壤相对含水率较为均匀无显著差异,240 m处理畦长畦首、畦中和畦尾的土壤相对含水率产生显著性差异;80和120 m处理夏玉米在畦首、畦中和畦尾灌水均匀度较为平均且无显著差异,240 m畦长处理差异较为显著,且80 m畦长处理产量分布最为平均、平均水分利用效率最高产量为1.021 839万kg/hm2,水分利用效率为25.12 kg/(hm2·mm)。综合夏玉米产量及其分布、水分利用效率等因素,在泾惠渠灌区80 m的灌溉畦长是兼顾节水高产的最适宜畦长,该结论以期为泾惠渠灌区大田作物高产节水提供一定理论依据。     

玉米茎结构参量与产量关联及最优化分析

高效高产农业是可持续生产的重点,为研究玉米茎结构参量综合利用效率及最优利用率产量提高幅度,本研究使用2015年北京市小汤山镇获取的玉米数据,将玉米茎结构参量作为输入指标,生物量、产量和经济系数为产出指标,运用数据包络分析探讨利用效率及最优利用效率。结果表明:‘京华8号’玉米茎结构参量利用效率都保持在0.7以上,仅67%达到最优利用效率水平;小喇叭口期和抽雄期茎结构参量利用效率中上部茎粗最差,下部茎粗最优但仍有提高潜力;玉米茎结构参量综合利用效率最优效率时资源充分利用产量有不同程度的提高,其中抽雄期提高产量最大为32%。玉米茎结构参量利用效率尚未达到最优,提高其利用效率尤其抽雄期可显著提高产量。